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KMID : 0379119890170030149
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Korean Journal of Mycology
1989 Volume.17 No. 3 p.149 ~ p.153
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Isolation of Aspergillus niger K-25 Producing Acid - stable α-amylase
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Abstract
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One strain of Aspergillus niger K-25 producing an acid-stable α-amylase was isolated from the soil. The optimum culture conditions were investigated. The production of the acidstable α-amylase was enhanced when the strain was incubated in a medium containing soluble starch 3.5%, peptone 2%, KH₂PO₄ 0.5%, MgSO₄·7H₂O 0.25% and FeCl₃ 1.0% at pH 3 for 7days. However, higher activity of acid-stable α-amylase was demonstrated on wheat bran culture. Amylase production was doubled when A. niger K-25 was incubated on the wheat bran supplemented with fumaric acid buffer (pH 3).
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